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Evaluation of nine microsatellite loci and misidentification paternity frequency in a population of Gyr breed bovines Braz. J. Vet. Res. Anim. Sci.
Curi,Rogério Abdallah; Lopes,Catalina Romero.
Paternity misidentification is harmful due to the reduction in annual genetic earnings of the population and because it endangers an efficient genetic improvement program. The objectives of the present study was to evaluate nine microsatellites in Paternity Testing and to investigate misidentification paternity frequency in families of Gyr breed bovines population. In the present experiment blood samples from forty Gir breed families ( bull / cow / calf ), registered pure breed in the Zebu Breeders Brazilian Association (ABCZ) were used. The most part of the microsatellites used in this work were recommended by the International Society of Animal Genetics (ISAG). The genomic DNA extraction was performed from whole blood samples. The microsatellites...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA; Microsatellites; Paternity; Bovine; Gyr.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000300004
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Individual contributions to pooled-milt fertilizations of silver catfish Rhamdia quelen Neotropical Ichthyology
Ribolli,Josiane; Zaniboni-Filho,Evoy.
Supplementary stocking of fish in natural environments is a way to mitigate or compensate for the changes imposed on wild populations by river damming. Since little is known about the genetic composition of the supplementary stocks obtained by pooled-milt fertilization, the aim of this study was to determine the individual contributions of male jundiá Rhamdia quelen to offspring. Sperm from four males were mixed using equal volume of sperm from each of the males to fertilize eggs from only one female, kept in three blend with six males and three females. The proportions of larvae sired by the different males were quantified using five polymorphic DNA microsatellite loci. Analysis of these loci allowed paternal determination of 84% of the progeny, at a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Jundiá; Milt pool; Paternity; Genetic diversity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400011
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Paternity testing and behavioral ecology: a case study of jaguars (Panthera onca) in Emas National Park, Central Brazil Genet. Mol. Biol.
Soares,Thannya Nascimento; Telles,Mariana P.C.; Resende,Lucileide V.; Silveira,Leandro; Jácomo,Anah Tereza A.; Morato,Ronaldo G.; Diniz-Filho,José Alexandre F.; Eizirik,Eduardo; Brondani,Rosana P.V.; Brondani,Claudio.
We used microsatellite loci to test the paternity of two male jaguars involved in an infanticide event recorded during a long-term monitoring program of this species. Seven microsatellite primers originally developed for domestic cats and previously selected for Panthera onca were used. In order to deal with uncertainty in the mother's genotypes for some of the loci, 10000 values of W were derived by simulation procedures. The male that killed the two cubs was assigned as the true sire. Although the reasons for this behavior remain obscure, it shows, in principle, a low recognition of paternity and kinship in the species. Since the two cubs were not very young, one possibility is that the adult male did not recognize the cubs and killed them for simple...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ecological stress; Infanticide; Jaguar; Microsatellites; Paternity.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400025
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